Transcript – Adaptive Assembly of Cotton Root Microbiome in Gel Microdroplet-Based Microfluidic Platform

Our project was focused on developing tools and resources to improve foundational knowledge of how cotton rise or volume, which includes the pathogen Šefčovič for changes dynamically over time in response to abiotic antibiotic factors and how we may be able to lead this adaptive assembly in our favor. We first sampled multiple soil samples and isolated bacteria exhibiting A.F. of four activity. We investigated the mode of communication between EFORE before and bacterial isolates, focusing on organic metabolites and volatile organic compounds. As shown in FIGURE 2, we learned that certain bacterial combinations communicate effectively through the use of Yosses. We studied cotton research field for Šefčovič, for inactive intensity in different cotton varieties in Figure 3, which also showed the correlation with environmental variations. Notably, we showed in Figure 4 that certain cotton varieties amplify inoculation. Over the course of the growing season, suggesting abiotic antibiotic factors are in play. Lastly, we also developed and tested a microfluidic system that can rapidly test a multitude of different experimental combinations as shown in Figure 5.

Our goal is to expand and further fine-tune this array to contain hundreds and perhaps thousands of chambers to perform high throughput phenotype screening for fungal and bacterial species in future microbiome research. This collaboration is providing foundational knowledge on important communication tools for fungal bacterial and bacterial association that can help elucidate the fundamental mechanisms that lead to the adaptive assembly of a tolerant rhizobia.