Transcript – Raman-Based Diagnostics of Libebacter Diseases in Tomatoes

These funds were used to launch a collaboration between Dzmitry Kurouski, located in the Department of Biochemistry and Biophysics, and Cecilia Tamborindeguy from the Department of Entomology, to study Raman based diagnostic of libebacter disease in tomatoes. Raman spectroscopy is a non-invasive and nondestructive analytical technique that can be used to probe structure and composition of analyzed samples. In this work, we show that Raman spectroscopy can be used for confirmatory, non-invasive and nondestructive diagnostics of candidates to be backtrace a.l effluvium limited gram-negative bacterium that infects crops worldwide. Specifically, this bacterium infects tomatoes, causing yellowness of leaves and essentially the death of the plant. Currently, the disease can be detected only with the use of polymerase chain reaction, which is time and labor consuming. Therefore, there is an urgent need for ultranet techniques that can be used for non-invasive and confirmatory diagnostics of bacter disease in tomatoes. In our study, we have grown tomatoes that were infected by two different haplotypes of bacter Haplotype A and Haplotype B, also known as A and B, and scanning the leaves with a handheld RAIMUND spectrometer, we were able to collect more than 150 spectra and with the use of Kema matrix, we were able to demonstrate that Raman spectroscopy can be used for highly accurate prediction of both Elizardo A and B by other means. It can be used to probe the presence of libebacter disease in tomatoes. It should be noted that plants did not exhibit any unusual symptoms. Therefore, we can talk about pre symptomatic disease. Diagnostic results of this paper were published in Planta in the Southern Twenty.